MyBioSource.com's PINK kinase Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: ELISA, and Western Blot. The PINK kinase Antibody was generated using PINK1 as the antigen and it reacts with Human.
Description
PINK1 (PTEN induced protein kinase 1) is a mitochondrial targeted serine/threonine kinase that promotes cell survival, particularly under conditions of oxidative/metabolic stress (1). The PINK1 gene encodes for a 70kDa protein that comprises a highly conserved kinase domain of the Ca2+/calmodulin family. Like most mitochondrial proteins, PINK1 is encoded on the nuclear genome and synthesized as a precursor polypeptide at cytosolic ribosomes (2). The catalytic domain of PINK1 is not closely related to other protein kinases and it possesses three unique insertions between the beta strands that make up the typical fold of the N-lobe of protein kinases (3). PINK1 contains a conserved C-terminal non-catalytic region of unknown function. PINK1 protein is cleaved upon entry into the mitochondria to produce two N-terminally truncated protein fragments that localize more to the cytosolic than to the mitochondrial fraction (1). PINK1 is targeted to its final mitochondrial localization in governed by specific signal sequences and accomplished by chaperone proteins and translocation machinery present in the mitochondrial membranes (2). The most common mitochondrial targeting signals are cleavable N-terminal extensions, which are initially recognized by the cytosol-exposed receptor proteins of the outer membrane (2). Missense mutations in PINK1 caused autosomal-recessive inherited Parkinson's disease (PD). The Parkinson disease-associated kinase PINK1 is targeted to mitochondria where it is thought to regulate mitochondrial quality control by promoting the selective autophagic removal of dysfunctional mitochondria.
PINK1- selective antibodies were generated against a peptide taken from the C-terminal of the PINK1 protein from region spanning 484-495 aa. The PINK1-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The anti- PINK1-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions